乙型肝炎病毒表面抗原的微团化及其免疫刺激复合物抗原的理化特性和免疫原性

本文用哺乳动物细胞系表达的乙型肝炎病毒表面抗原(HBsAg),制备了HBsAg的微团化(Micelle)和免疫剂激复合物(Immune-stimulating Complexes,简称ISCOMS)两种形式的抗原。在电镜下观察,微团化抗原是由球形亚单位颗粒组成直径100～150nm的较原颗粒大得多的大颗粒,在蔗糖中的浮力密度为1.24g/ml;而ISCOMS在电镜下为直径30～40nm左右稍大于原颗粒的多面体形态颗粒。SDS-PAGE分析表明,这两种形式的颗粒都是由HBsAg的P23和GP27蛋白所组成。 小鼠免疫接种结果显示,ISCOMS的免疫原性优于微团化抗原,后者又优于原22nm HBsAg颗粒。在抗体产生的速度和强度上,ISCOMS组显著优于微团化抗原组,而微团化抗原组略优于22nm HBsAg组。 ISCOMS的免疫性强,抗体产生早,强度高,又易于制备,而且不需要使用氢氧化铝胶佐剂,有可能发展成为一种新一代的乙型肝炎疫苗。

IMMUNOGENICITY OF MICELLE FORM AND IMMUNE-STIMULATING COMPLEXES PREPARED FROM HEPATITIS B VIRUS SURFACE ANTIGEN

We report here the preparation of micelle form and Immune-stimulating Complexes ( ISCOMS ) from recombinant HBsAg expressed by the mammalian cell line and studies on their physico-chemical and immu nological properties.The buoyant density of the micelles in sucrose was about 1.24g/ml as compared with a density of 1.2lg/ml for recombinant HBsAg. Electron microscopy showed that the micelles are spheroid particles with a diameter of 100-150nm and the ISCOMS to be cage like multiform particles with a diameter of 30-40nm. SDS-PAGE indicated both the micelles and ISCOMS consisted of GP27 and P23.The immunogenicity of ISCOMS was compared with that of micelle form and intact 22-nm HBsAg in mice. The result revealed that ISCO MS had higher immunogenicity than the micelle form which in turn was slightly better than the 22nmHBsAg.