In vitro propagation of Coleus forskohlii Briq., a threatened medicinal plant |
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Authors: | Neelam Sharma K. P. S. Chandel V. K. Srivastava |
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Affiliation: | (1) National Plant Tissue Culture Repository, National Bureau of Plant Genetic Resources, Pusa Campus, 110012 New Delhi, India;(2) Chemistry Unit, National Bureau of Plant Genetic Resources, Pusa Campus, 110012 New Delhi, India |
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Abstract: | In vitro clonai multip1ication of Coleus forskahlii Briq a threatened plant, has been achieved on MS medium supplemented with Kn (2.0 mg/l) and IAA (1.0 mg/l) using nodal segments as explants, Shoots multiplied at a rate of 12 — fold every six weeks. Rooting was achieved upon transfer of shoots onto MS medium containing IAA (1.0 mg/l). The micropropagated plants were successfully established under field conditions. Forskolin content in tubers of plants obtained by micropropagation was found to be 0.1%, the same as that found in wild plants. This micropropagation procedure should be useful for conservation as well as production of this important plantAbbreviations BAP 6-benzylaminopurine - IAA indole-3-acetic acid - IBA indole-3-butyric acid - Kn Kinetin - MS Murashige and Skoog (1962) basal medium - NAA -naphthalene acetic acid |
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