Comparison of clastogenic effects of inorganic selenium salts in mice in vivo as related to concentrations and duration of exposure |
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Authors: | Sonali Biswas Geeta Talukder Archana Sharma |
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Institution: | (1) Department of Pathology, Vivekananda Institute of Medical Sciences, 99, Sarat Bose Road, Calcutta, 700 026, India;(2) Centre for Advanced Studies in Cell and Chromosome Research, Department of Botany, University of Calcutta, 35, Ballygange Circular Road, Calcutta, 700 019, India |
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Abstract: | Inorganic selenium compounds in the diet have been known to protect against cancer in laboratory animals, but were harmful in high concentrations. In the present work, the relative effects of two salts, sodium selenite and sodium selenate, administered to mice in vivo, in different concentrations and durations of exposure, were compared. Aqueous solutions of each salt (7, 14, 21 and 28 mg Kg–1 bw) were fed by gavaging to mice matched in age and sex. The animals were sacrificed at intervals of 6, 12, 18 and 24 h and chromosome preparations were made following the usual schedule of colchicine-hypotonic-fixative-airdrying-Giemsa staining. The endpoints screened were chromosomal aberrations (CA) and damaged cells (DC). Both salts affected chromosome structure and spindle formation, sodium selenite being more cytotoxic than sodium selenate. The frequencies of aberrations induced were directly proportional to the concentrations used and duration of exposure. |
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Keywords: | selenium clastogenicity chromosomal aberrations |
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