The effects of water temperature and hormone treatments on circulating LH and ovulation in tench (Tinca tinca) |
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| Authors: | Peter Podhorec Magdalena Socha Miroslawa Sokolowska-Mikolajczyk Tomas Policar Viktor W. Svinger Grzegorz Gosiewski Antonin Kouba Jan Kouril |
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| Affiliation: | 1. Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Zatisi 728/II, 389 25, Vodnany, Czech Republic
2. Department of Ichthyobiology and Fisheries, University of Agriculture, ul. Prof. T. Spiczakowa 6, 30-199, Krakow-Mydlniki, Poland
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| Abstract: | The effectiveness of three hormone treatments commonly used for artificial reproduction of tench was evaluated under three thermal regimes, with focus on the need for dopamine antagonist. Mature females were divided into nine groups (n?=?8) and gradually exposed, over a 24?h period, to three temperature regimes: cold (18.1?±?0.02?°C), optimal (22.05?±?0.03?°C), and warm (26.3?±?0.01?°C). Each temperature regime comprised three experimental groups injected with one of three hormone treatments: carp pituitary extract (CPE; 3?mg?kg?1); [D-Arg6, Pro9, NEt]-sGnRH (10???g?kg?1); and [D-Arg6, Pro9, NEt]-sGnRH (10???g?kg?1)?+?metoclopramide (20?mg?kg?1) (combined treatment). No differences were found between ovulation induction (ovulation rate????75?%) with sGnRHa alone and with the combined treatment; whereas CPE at cold and warm water temperatures was significantly less effective (P?P?
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