Confocal microscopy of cells implanted into tissue blocks: Cell migration in long-term histocultures |
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| Authors: | Leonid B Margolis Svetlana E Glushakova Boris A Baibakov Carlos Collin Joshua Zimmerberg |
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| Institution: | (1) Laboratory of Theoretical and Physical Biology, National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bldg. 10, Room 10D14, 20892 Bethesda, Maryland;(2) Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia;(3) Office of the Director, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 20892 Bethesda, Maryland |
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| Abstract: | Summary In three-dimensional tissues in vivo, cells find themselves in a unique, heterogeneous microenvironment among various cellular
and noncellular elements. Cells are greatly affected by and contribute to their physical and chemical microenvironments. However,
live cells are currently studied predominantly in homogeneous monolayer cultures where newly established contacts might be
fundamentally different from contacts in vivo. Several systems have been suggested to simulate the three-dimensional environment
of real tissue. In this report, we describe a new system for studying cell behavior inside real tissues in vitro. By fluorescently
labeling mouse tumor cells, then implanting them into cultured tissue blocks (histocultures), we have observed cellular location
and followed their locomotion, within tissues in vitro for days. We discuss the potential of the described system for studying
different aspects of cell behavior in a nativelike microenvironment. |
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| Keywords: | tumor invasion lung tissue in vitro |
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