Construction of an <Emphasis Type="Italic">Aspergillus oryzae</Emphasis> cell-surface display system using a putative GPI-anchored protein |
| |
Authors: | Takashi Adachi Junji Ito Kouji Kawata Masahiro Kaya Hiroki Ishida Hiroshi Sahara Yoji Hata Chiaki Ogino Hideki Fukuda Akihiko Kondo |
| |
Institution: | (1) Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe Hyogo, 657-8501, Japan;(2) Organaization of Advanced Science and Technology, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe Hyogo, 657-8501, Japan;(3) Research Institute, Gekkeikan Sake Co., Ltd., 300 Katahara-cho, Fushimi-ku Kyoto, 612-8361, Japan |
| |
Abstract: | A novel cell-surface display system was constructed in Aspergillus oryzae. Each of the five genes encoding the putative cell-wall-localized protein from the A. oryzae genome was cloned and these cell-surface anchor functions were examined by fusion to the C-terminal of the green fluorescent
protein (GFP). Using the MP1 and CWP proteins as anchor proteins, GFP signals were strongly observed on the cell surface of
recombinant A. oryzae. When these proteins were used as anchor proteins for cell-surface display of β-glucosidase from A. oryzae, enzyme activity was detected on the cell surface. In particular, β-glucosidase activity of recombinant A. oryzae using MP1, a putative glycosylphosphatidylinositol (GPI) anchor protein was higher than CWP. Based on these results, it was
concluded that the MP1 protein can act as a GPI-anchor protein in A. oryzae, and the proposed cell-surface display system using MP1 allows for the display of heterogeneous and endogenous proteins. |
| |
Keywords: | Aspergillus oryzae Cell-surface display GPI-anchored protein |
本文献已被 PubMed SpringerLink 等数据库收录! |
|