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| 根癌农杆菌介导丝状真菌遗传转化的研究进展 | |
| 引用本文： | 黄亚丽,潘玮,蒋细良,郭平,田云龙,李记鹏,朱昌雄. 根癌农杆菌介导丝状真菌遗传转化的研究进展[J]. 生物技术通报, 2007, 0(3): 111-114 |
| 作者姓名： | 黄亚丽 潘玮 蒋细良 郭平 田云龙 李记鹏 朱昌雄 |
| 作者单位： | 1. 中国农业科学院环境与农业可持续发展研究所,北京,100081;河北省生物研究所,石家庄,050081 2. 中国农业科学院环境与农业可持续发展研究所,北京,100081 3. 华北油田公司,任丘,062552 |
| 摘 要： | 根癌农杆菌介导的丝状真菌遗传转化是近年建立起来的一种新方法,该方法和以往的真菌转化体系相比具有转化方法简单、材料易得、效率高以及转化子中T-DNA单拷贝插入比例高等特点。就根癌农杆菌转化的丝状真菌种类、转化的具体过程以及影响转化效率的因素等方面进行了综述,并展望了该方法的应用前景。 |
| 关 键 词： | 根癌农杆菌 丝状真菌 遗传转化 |
| 修稿时间： | 2006-12-27 |
Transformation in Filamentous Fungi Mediated by Agrobacterium tumefaciens |
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| Huang Yali,Pan Wei,Jiang Xiliang,Guo Ping,Tian Yunlong,Li Jipeng,Zhu Changxiong. Transformation in Filamentous Fungi Mediated by Agrobacterium tumefaciens[J]. Biotechnology Bulletin, 2007, 0(3): 111-114 | |
| Authors: | Huang Yali Pan Wei Jiang Xiliang Guo Ping Tian Yunlong Li Jipeng Zhu Changxiong |
| Affiliation: | 1Intitute of Agricuhural Environment and Sustainable Developrnent,CAAS,Beijing 100081;2Biological Institute of Hebei Province,Shijiazhuang 050081;3North China Oil Field Company,Renqiu 062552 |
| Abstract: | Agrobacterium tumefaciens mediated transformation(ATMT)was newly used to transform filamentous fungi.Because of the simplicity and efficiency of transformation via A.tumefaciens,it is relatively easy to generate a large number of stable transformants.In combination with the finding that the T-DNA integrates randomly and predominantly as a single copy,AMT is well suited to perform insertional mutagenesis in fungi.This paper summarized the advancement of ATMT and discussed the future application of this technology in filamentous fungi. |
| Keywords: | T-DNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |