The effect of membrane preparation and cellular maturation on human erythrocyte adenylate cyclase |
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Authors: | Masanori Nakagawa Joseph Willner Cesare Cerri Patricia Reydel |
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Institution: | Department of Neurology, Columbia University College of Physicians and Surgeons, 630 W. 168th Street, New York, NY 10032 U.S.A. |
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Abstract: | We found that adenylate cyclase activity of human erythrocytes is potentially labile during isolation of their plasmalemma. Addition of 1 mM EGTA to solution used to remove hemoglobin from lysed cells protected activity. Human erythrocyte adenylate cyclase is minimally activated by catecholamines, in the absence or presence of exogenous guanyl nucleotide, but substantially by 5′-guanylyl imidodiphosphate or sodium fluoride and concentration-dependently by Mg2+ or Mn2+. Basal catalytic activity is an age-dependent component of the human erythrocyte; 5′-guanylyl imidodiphosphate- or fluoride-activated activities decline with cellular maturation proportionally to the decrease in basal activity. |
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Keywords: | Adenylate cyclase Membrane isolation Erythrocyte membrane Cell maturation |
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