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DNA from Serratia marcescens confers a hydrophobic character in Escherichia coli |
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| Authors: | Ronit Bar Ness-Greenstein Mel Rosenberg R.J. Doyle Nachum Kaplan |
| Affiliation: | The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel-Aviv University, 69978 Tel-Aviv, Israel; Department of Human Microbiology, Sackler Faculty of Medicine, Tel-Aviv University, 69978 Tel-Aviv, Israel; Department of Microbiology / Immunology, University of Louisville, Louisville, KY, USA; Department of Molecular Microbiology and Biotechnology, Tel-Aviv University, 69978 Tel-Aviv, Israel |
| Abstract: | Abstract In order to determine whether hydrophobic surface properties of Serratia marcescens can be transferred to Escherichia coli , E. coli DH5α cells were transformed by DNA fragments from S. marcescens RZ. Fifteen-hundred E. coli transformants were screened for adhesion to hexadecane and polystyrene. One transformant exhibited increased adhesion to hexadecane droplets, as well as altered kinetics of aggregation in the presence of ammonium sulfate. Western colony blotting revealed that antibodies raised against S. marcescens RZ recognized components) on the transformant outer surface. |
| Keywords: | Serratia marcescens Hydrophobin Cell surface hydrophobicity Hexadecane Ammonium sulfate aggregation Escherichia coli |