cDNA cloning, characterization, and brain region-specific expression of a neuromedin-B-preferring bombesin receptor. |
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| Authors: | E Wada J Way H Shapira K Kusano A M Lebacq-Verheyden D Coy R Jensen J Battery |
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| Institution: | Laboratory of Neurochemistry, National Institute of Neurological Disorders and Stroke, National Institutes of Health Bethesda, Maryland 20892. |
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| Abstract: | Recent binding studies in the central nervous system and other tissues provide evidence that the mammalian bombesin-like peptides, gastrin-releasing peptide (GRP) and neuromedin-B (NMB), exert their numerous physiological effects through at least two different receptors. We describe the structure and expression of a cloned NMB-preferring bombesin receptor (NMB-R) with properties distinct from a GRP-preferring bombesin receptor (GRP-R) reported previously. In particular, the NMB-R shows higher affinity binding to NMB than to GRP in BALB 3T3 fibroblasts expressing the cloned NMB-R. The distinct regional distribution of NMB-R and GRP-R mRNA in the brain suggests that both bombesin receptor subtypes play independent roles in mediating many of the dramatic effects of bombesin-like peptides in the central nervous system. |
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