A new method for selective localization of flavan-3-ols in plant tissues involving glycolmethacrylate embedding and microwave irradiation. |
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Authors: | M Gutmann W Feucht |
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Affiliation: | Institut für Pflanzenbau, Lehrstuhl für Obstbau, Technische Universit?t München, Federal Republic of Germany. |
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Abstract: | A method for selective staining of flavan-3-ols in plant tissues fixed with glutaraldehyde is given. The use of glycolmethacrylate as embedding medium allows the sulphuric acid-containing staining solution to be heated without destroying the fine structure of the tissue. The distribution of flavan-3-ols and proanthocyanidins in different plant tissues is discussed. |
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