DNA ploidy of human breast cancer |
| |
Authors: | G Del Bino R Silvestrini M R Zucconi G Marchese B Valentinis G Di Fronzo S Andreola |
| |
Affiliation: | Istituto Nazionale per lo Studio e la Cura dei Tumori, Milano, Italy. |
| |
Abstract: | Ploidy was determined on 663 resectable primary tumors from untreated patients. Nuclei obtained by mechanical disaggregation of frozen tissue were stained with propidium iodide and analysed in a FACS IV. Aneuploidy was detected in 73% of cases. It was not significantly related to nodal involvement or tumor size, although the highest frequencies were observed in large tumors (88%) or with more than 10 positive nodes (77%). Aneuploidy was more frequently observed in ductal infiltrating (81%) than in lobular histology and in tumors lacking both progesterone and estrogen receptors (85%). Analysis of ploidy in primary and synchronous lymph node metastases from the same patient showed a high agreement rate (90%) of DNA patterns simply defined as diploid or aneuploid. However, differences in DNA stemlines and DNA indices between the two synchronous lesions from the same patient were a rather frequent event. |
| |
Keywords: | |
|
|