A sensitive assay for allantoin |
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| Authors: | John Abraham Fiorindo A. Simeone Robert W. Hopkins |
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| Affiliation: | 1. Division of Biological and Medical Sciences of Brown University, Providence, Rhode Island, USA;2. the Department of Surgery, The Miriam Hospital, Providence, Rhode Island, USA |
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| Abstract: | Allantoin is separated by thin-layer chromatography (TLC) and sprayed with an acidic solution ofp-dimethylaminobenzaldehyde. The yellow color produced is read in a densitometer and compared with that of a standard. The lower limit of quantitation is 0.1 μg per spot (0.5 mg/100 ml). The method can be utilized for the estimation of allantoin in serum, lymph, and urine. |
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