Effect of interleukin-1 receptor antagonist (IL-1RA) on histamine and serotonin release by rat basophilic leukemia cells (RBL-2H3) and peritoneal mast cells |
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Authors: | Pio Conti William Bouchet Claudio Feliciani Sandra Mammarella Leena Kudchadker Renato C Barbacane Marcella Reale Iman Haggag Gilles Bruneau Jean Thibault Theoharis C Theoharides |
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Institution: | (1) Immunology Division, Institute of Experimental Medicine, University of Chieti, Via dei Vestini, 66100 Chieti, Italy;(2) Dermatology Division, University of Chieti, Via dei Vestini, 66100 Chieti, Italy;(3) Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, 136 Harrison Avenue, 02111 Boston, Massachusetts, USA;(4) Laboratoire de Biochimie Cellulaire, College de France, Paris, France;(5) Cattedra di Immunologia, Istituto di Medicina Sperimentale, Facoltà di Medicina, Universià di Chieti, Via dei Vestini, 66 100 Chieti, Italy |
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Abstract: | Recently, it has been appreciated that cultured mast cells are significant sources of cytokines. However, the role of interkeukin-1 (IL-1) on mast cells and/or basophil degranulation is still unclear. In this report we provide evidence that rat basophilic leukemia cells (RBLC) cultured with a natural inhibitor of IL-1, interleukin-1 receptor antagonist (IL-1RA) (500 ng/ml) for 48 h, strongly inhibited the spontaneous release of serotonin (5HT) and histamine (from 22.50 to 43.49%), compared to untreated cells (control). When IL-1RA-treated and untreated RBLC were stimulated with a secretagogue (anti-IgE), no difference was found in the percent of 5HT and histamine release. Moreover, in another set of experiments using rat peritoneal mast cells (RPMC) treated and untreated with IL-1RA, we found that IL-1RA did not affect the release of 5HT or histamine, even when the secretagogue anti-IgE or compound 48/80 (C48/80) were used. The present studies describe an additional biological activity of IL-1RA, inhibiting histamine and 5HT release from RBLC cultures.Abbreviations IL-1
interleukin-1
- RA
receptor antagonist
- 5HT
serotonin
- RBLC
rat basophilic leukemia cells
- RPMC
rat peritoneal mast cells
- IgE
immunoglobulin E
- Fc
immunoglobulin E receptor
- CPM
counts per minute
- BSA
bovine serum albumin
- C48/80
compound 48/80
- TNF
tumor necrosis factor |
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Keywords: | interleukin-1 receptor antagonist histamine serotonin mast cells rat basophilic leukemia cells |
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