Development of human lactic acid (LAB) gastrointestinal microbiota in a Greek rural population |
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Authors: | Dimitrios Vassos Vassiliki Maipa Chrysa Voidarou Athanasios Alexopoulos Eugenia Bezirtzoglou |
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Affiliation: | (1) Department of Hygiene, Medical School, University of Ioannina, GR45 100 Ioannina, Greece;(2) Laboratory of Animal Health and Infectious Diseases, Department of Animal Production, Technological Educational Institute of Epirus, GR47 100 Arta, Greece;(3) Laboratory of Food Microbiology, Biotechnology and Hygiene, Faculty of Agricultural Development, Democritus University of Thrace, GR28 200 Orestiada, Greece |
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Abstract: | In order to investigate the Lactic Acid Bacteria (LAB) of the gut, fecal samples were collected and analyzed from 120 healthy Greek volunteers ranging from age 1 to age 85, all of whom declared daily consumption of local fermented dairy products. LAB strains were isolated using selective media under aerobic or anaerobic conditions. Identification of the isolates was based on their growth patterns, morphological characteristics, and carbohydrate fermentation profiles. There was no significant difference in the abundance of Lactobacillus brevis, Lactococcus lactis subsp. cremoris, Lactococcus lactis, Lactobacilus paracasei and Bifidobacterium sp., in all samples. Lactobacillus fermentum, Lactobacillus plantarum, Lactococcus casei, Lactococcus pentosus, Lactococcus lactis subsp. lactis, Lactococcus delbrueckii subsp. lactis, Enterococcus casseliflavus, Enterococcus faecalis, Enterococcus faecium, Enterococcus avium and Leuconostoc sp. were also recovered, mainly from the adults and elders rather than the children’s group. Despite the above differences in LAB species observed mostly between the younger and the other two age groups, differences were also observed within all groups, indicating that healthy subjects of all ages are colonized by a diverse lactoflora in terms of total or dominant species. |
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Keywords: | Lactobacillus Lactococcus C. perfringens Fecal Human gastrointestinal flora Lactic acid bacteria |
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