大鼠骨骼肌快肌肌钙蛋白T的同工型

骨骼肌快肌的收缩主要是由钙离子通过肌钙蛋白所调节控制。这些肌钙蛋白位于肌纤维之中。肌蛋白包括肌钙蛋白T、肌钙蛋白C、肌钙蛋白I。采用双向聚丙烯酰胺凝胶电泳和免疫学技术，对大鼠胚胎、新生大鼠和成年大鼠的骨能肌快肌肌钙蛋白T的同工型进行了研究。在成年大鼠的骨能肌快肌中，发现了10种肌钙蛋白T同工型。在大鼠胚胎和新生大鼠的骨能肌中，发现了7种肌钙蛋白T同工型。作为不同动物、不同发育阶段和不同组织发育的特殊标记，这些肌钙蛋白T同工型具有重要意义动物学报49(3)：362—369，2003]。

Troponin T isoforms in rat fast skeletal muscles

The relaxation and contraction in vertebrate skeletal muscle are regu lated by Ca2+ through troponin and tropomyosin, which are located in the thin filament. Troponin is composed of three components, troponins C, I and T. Rat trop onin T isoforms were examined in fetal, neonatal and adult rat skeletal muscles using two-dimensional polyacrylamide gel electrophoresis and immunology. Ten kinds of isoforms were discovered in adult rat fast skeletal muscles. Seven kinds of isoforms were found in fetal and neonatal rat skeletal muscles. These isoforms of troponin T are valuable in the development of specific markers in different tissues in different development stages and in different animals. The new TnT is oforms in rat skeletal muscles described in this paper will be useful in future research on specific markers.