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| 大鼠脑线粒体NOS及L—Arg转运的生化特性 | |
| 作者姓名： | Cao J Wang L Zhao BL Chen QT Qi YF Tang CS |
| 作者单位： | 1. 北京大学第一医院心血管研究所;宁夏医学院病理生理教研室, 银川 750004 2. 北京大学第一医院神经研究中心, 北京 100034 3. 中国科学院生物物理研究所, 北京 100101 4. 北京大学第一医院心血管研究所 |
| 基金项目： | This work was supported by National Major Basic Research Program (G2000056905). |
| 摘 要： | 测定分离纯化的大鼠脑线粒体(mitochondria,Mt)L-精氨酸(L-arginine,L-Arg)/一氧化氮合酶(nitricoxidesynthase,NOS)/NO系统,L-Arg转运和NOS的活性。结果显示正常大鼠脑Mt膜上存在高亲和、低转运、可饱和的L-Arg转运体。最大转运速率Vmax为5.87±0.46nmol/mgpro·min |
| 关 键 词： | 线粒体 一氧化氮 L－精氨酸 一氧化氮合酶 脑 |
| 修稿时间： | 2000年10月27 |
The biochemical properties of L-arginine/nitric oxide pathway in rat brain mitochondria |
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| Cao J,Wang L,Zhao BL,Chen QT,Qi YF,Tang CS.The biochemical properties of L-arginine/nitric oxide pathway in rat brain mitochondria[J].Acta Physiologica Sinica,2001,53(4):261-264. | |
| Authors: | Cao J Wang L Zhao B L Chen Q T Qi Y F Tang C S |
| Institution: | Institute of Cardiovascular Diseases, the First Hospital, Peking University, Beijing 100034. |
| Abstract: | Biochemical property of L-arginine (L-Arg)/nitric oxide synthase (NOS)/nitric oxide (NO) system was observed in rat brain mitochondria. The results showed that there existed L-Arg transporters of high-affinity, low-transport and saturability in mitochondria membrane of normal rat brain. The maximum transport velocity (V(max)) and Michaelis constant (Km) were 5.87+/ -0.46 nmol/mg pro x min(-1) and 7.8+/- 0.56 micromol/L respectively. In normal condition, endothelium-type NOS was expressed in the mitochondria of rat brain, with its V(max) and Km being 2.7+/-0.3 nmol/mg pro x min(- 1) and 20.85+/-3.27 micromol/L, respectively. NO production was increased in a time-dependent manner and reached a maximum value at 60 min. These results suggest that the L-Arg transporters in MT membrane of normal rat brain were similar with Y(+) type amino acid transporters in cellullar membrane. Furthermore the NOS of mitochondria belongs to eNOS, whereas the L-Arg/NOS/NO system may be a source of NO. |
| Keywords: | mitochondria nitric oxide L arginie transport nitric oxide synthase |
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