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| 拟南芥转录因子CBF1和CBF4基因的克隆与序列分析 | |
| 引用本文： | 徐春波,王勇,杨志如,赵海霞,李兴酉. 拟南芥转录因子CBF1和CBF4基因的克隆与序列分析[J]. 生物技术通报, 2010, 0(2) |
| 作者姓名： | 徐春波 王勇 杨志如 赵海霞 李兴酉 |
| 作者单位： | 1. 中国农业科学院草原研究所,呼和浩特,010010 2. 内蒙古农业大学生态环境学院,呼和浩特,010018 3. 内蒙古鄂尔多斯市水利勘测设计院,东胜,017000 |
| 基金项目： | 中央级公益性科研院所基本科研业务费专项资会资助项目 |
| 摘 要： | 利用聚合酶链式反应技术(PCR)从拟南芥(Arabidopsis thaliana)植株中扩增出CBF1和CBF4基因,并将其连接到pGEM-Tvector载体上。PCR和酶切鉴定后进行基因测序,结果表明所克隆的CBF1和CBF4基因序列与GenBank中基因序列同源性分别可达99.84%和99.41%,说明这两个片段确为拟南芥CBF1和CBF4基因。 |
| 关 键 词： | 拟南芥PCR 克隆 |
Cloning and Sequences Analysis of Transcription Factor CBF1 and CBF4 Gene in Arabidopsis thaliana |
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| Xu Chunbo,Wang Yong,Yang Zhiru,Zhao Haixia,Li Xingyou. Cloning and Sequences Analysis of Transcription Factor CBF1 and CBF4 Gene in Arabidopsis thaliana[J]. Biotechnology Bulletin, 2010, 0(2) | |
| Authors: | Xu Chunbo Wang Yong Yang Zhiru Zhao Haixia Li Xingyou |
| Affiliation: | Xu Chunbo1 Wang Yong2 Yang Zhiru3 Zhao Haixia1 Li Xingyou1(1Grassl, Research Institute of Chinese Academy of Agricultural Sciences,Huhhot 010010,2College of Ecology , Environment Inner Mongolia Agricultural University,Huhhot 010018,3 Water Conservancy Survey , Design Institute in Erdos City,Dongsheng 017000) |
| Abstract: | CBF1 and CBF4 genes were obtained by PCR technique from the plants of Arabidopsis thaliana and then connected those into the pGEM-T vector.PCR,Restriction enzyme digestion and Sequencing results showed that the cloned gene sequences of CBF1 and CBF4 have highly homologous with GenBank,reached 99.84%,99.41% Respectively.Thus,the two fragments were proved as CBF1 and CBF4 gene fragments |
| Keywords: | CBF1 CBF4 CBF1 CBF4 Arabidopsis thaliana PCR Clone |
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