Uncoating ATPase is a member of the 70 kilodalton family of stress proteins |
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| Authors: | T G Chappell W J Welch D M Schlossman K B Palter M J Schlesinger J E Rothman |
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| Institution: | 1. Key Laboratory of Glycoconjugate Research Ministry of Public Health, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, PR China;2. Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, PR China;3. Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, PR China;1. l''institut du thorax, Inserm, CNRS, Université de Nantes, Nantes, France;2. l''institut du thorax, Inserm, CNRS, Université de Nantes, CHU Nantes, Nantes, France;3. ER045, PRASE, Laboratory of stem cells, Lebanese university, Beirut, Lebanon;4. Centre d''études d''agents Pathogènes et Biotechnologies pour la Santé, CNRS, Université de Montpellier, Montpellier, France;1. Center for AIDS Health Disparities Research, Department of Microbiology and Immunology, Meharry Medical College, Nashville, TN 37208, USA;2. National Engineering Laboratory for AIDS Vaccine, School of Life Science, Jilin University, Changchun, Jilin Province 130000, People''s Republic of China;1. ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville Q4811, Australia;2. Experimental Marine Ecology Laboratory, Department of Biological Sciences, National University of Singapore, 117543, Singapore;3. Center for Genomics and Systems Biology, New York University, Abu Dhabi, PO Box 129188, Abu Dhabi, United Arab Emirates |
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| Abstract: | The synthetic peptide, VGIDLGTTYSC, derived from the heat shock-induced genes human hsp70, Drosophila hsp70, S. cerevisiae YG100, and E. coli dnaK, elicited antibodies that recognized two constitutive proteins in bovine extracts. One of these proteins, 71 kd, has previously been identified as uncoating ATPase, an enzyme that releases clathrin from coated vesicles. This immunological data complemented the result that uncoating ATPase was indistinguishable from the constitutive mammalian 71 kd stress protein by either partial proteolytic mapping or two-dimensional gel analysis. In addition, affinity-purified uncoating ATPase antibodies recognize proteins in yeast identified as the gene products of the heat shock or heat shock cognate genes YG100 and YG102. The results show that uncoating ATPase is a member of the 70 kd heat shock protein family. |
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