Aberrant expression of cytoskeleton proteins in hippocampus from patients with mesial temporal lobe epilepsy |
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Authors: | J W Yang T Czech M Felizardo C Baumgartner G Lubec |
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Institution: | (1) Department of Pediatrics, Medical University of Vienna, Vienna, Austria;(2) Department of Neurosurgery, Medical University of Vienna, Vienna, Austria;(3) Section of Clinical Epilepsy Research, Department of Neurology, Medical University of Vienna, Vienna, Austria |
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Abstract: | Summary. Mesial temporal lobe epilepsy (MTLE), the most common form of epilepsy, is characterised by cytoarchitectural abnormalities
including neuronal cell loss and reactive gliosis in hippocampus. Determination of aberrant cytoskeleton protein expression
by proteomics techniques may help to understand pathomechanism that is still elusive. We searched for differential expression
of hippocampal proteins by an analytical method based on two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry
unambiguously identifying 77 proteins analysed in eight control and eight MTLE hippocampi. Proteins were quantified and we
observed 18 proteins that were altered in MTLE. Cytoskeleton proteins tubulin α-1 chain, β-tubulin, profilin II, neuronal
tropomodulin were significantly reduced and one actin spot was missing, whereas ezrin and vinculin were significantly increased
in MTLE. Proteins of several classes as e.g. antioxidant proteins (peroxiredoxins 3 and 6), chaperons (T-complex protein 1-α,
stress-induced-phosphoprotein 1), signaling protein MAP kinase kinase 1, synaptosomal proteins (synaptotagmin I, α-synuclein),
NAD-dependent deacetylase sirtuin-2 and 26S protease regulatory subunit 7 protein, neuronal-specific septin 3 were altered
in MTLE. Taken together, the findings may represent or lead to cytoskeletal impairment; aberrant antioxidant proteins, chaperons,
MAP kinase kinase 1 and NAD-dependent deacetylase sirtuin-2 may have been involved in pathogenetic mechanisms and altered
synaptosomal protein expression possibly reflects synaptic impairment in MTLE.
J. W. Yang and T. Czech have equally contributed to the paper. |
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Keywords: | : Antioxidant – Chaperone – Cytoskeleton – Hippocampus – Mesial temporal lobe epilepsy (MTLE) |
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