| 温度梯度凝胶电泳技术及应用 |
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| 引用本文： | 陈汉奎,冯忻.温度梯度凝胶电泳技术及应用[J].生物化学与生物物理进展,1999,26(3):297-299. |
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| 作者姓名： | 陈汉奎 冯忻 |
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| 作者单位： | 广州市肿瘤医院,广州,510095 |
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| 摘 要： | 温度梯度凝胶电泳（TGGE）是一种用于检测核酸序列变异和点突变的电泳方法.该法利用不同构象的核酸分子具有不同的变性温度（Tm)来进行分离.TGGE方法具有分辨能力高、重复性好和节省时间的特点，可广泛应用于分子生物学研究领域.
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| 关 键 词： | 温度梯度凝胶电泳 变性温度 序列变异 点突变 |
| 收稿时间： | 1998/9/14 0:00:00 |
| 修稿时间： | 2/1/1998 12:00:00 AM |
The Technique of Temperature Gradient Gel Electrophoresis and Its Applications |
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| CHEN Han-Kui and FENG Xin.The Technique of Temperature Gradient Gel Electrophoresis and Its Applications[J].Progress In Biochemistry and Biophysics,1999,26(3):297-299. |
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| Authors: | CHEN Han-Kui and FENG Xin |
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| Abstract: | Temperature gradient gel electrophoresis (TGGE) is a new and powerful electrophoresis method for separation of nucleic acids (DNA and RNA) and analysis of sequence variations. The TGGE method uses the melting temperature(Tm) as an important parameter to identify DNA, which differs in sequence among a mixture of molecules of the same size but different conformation. With the advantages of high-resolution capability, high reproducibility and timesaving process, the TGGE technique has been applied broadly in the field of molecular biology. |
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| Keywords: | temperature gradient gel electrophoresis melting temperature sequence variation point mutation |
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