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New acidic chitinase isoforms induced in tobacco roots by vesicular-arbuscular mycorrhizal fungi
Authors:Eliane Dumas-Gaudot  Valentin Furlan  Jean Grenier  Alain Asselin
Institution:(1) Laboratoire de Phytoparasitologie, INRA-CNRS, Station de Génétique et d'Amélioration des Plantes, INRA, F-21034 Dijon Cedex, France;(2) Station de Recherches, Agriculture Canada, 2560, Boulevard Hochelaga, G1V 2J3 Sainte-Foy, Quebec, Canada;(3) Département de Phytologie, Faculté des Sciences de l'Agriculture et de l'Alimentation, Université Laval, G1K 7P4 Quebec, Canada
Abstract:Summary Chitinase activities have been compared in tobacco roots (Nicotiana tabacum cv. Xanthi nc) infected by the pathogenic fungus Chalara elegans or three species of vesicular arbuscular mycorrhizal (VAM) fungi: Glomus versiforme, G. intraradix and G. fasciculatum, using native polyacrylamide gel electrophoresis (PAGE). All previously known acidic chitinase isoforms were stimulated in roots by the pathogenic fungus and by the VAM fungi, while two new acidic chitinase isoforms were specifically induced in response to the endomycorrhizal association. After separation in sodium dodecyl sulphate polyacrylamide denaturing gels (SDS-PAGE) under non-reducing conditions, the estimated apparent molecular mass for these additional acidic chitinase isoforms from VAM-colonized samples was 33 kDa, compared to 30 kDa for the main activity stimulated in C. elegans-infected root extracts.
Keywords:Tobacco roots  Chalara elegans  Glomus spp  Chitinases  PR proteins
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