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The detection of 5-lipoxygenase and cyclo-oxygenase products in sputum of patients with chronic bronchitis and bronchiectasis
Authors:J.T. Zakrzewski   N.C. Barnes   P.J. Piper  J.F. Costello
Affiliation:2. Department of Thoracic Medicine King''s College School of Medicine and Dentistry Denmark Hill London SE5 8RX, UK;1. Department of Pharmacology Hunterian Institute Royal College of Surgeons of England London WC2A 3PN, UK;1. Division of Infectious Disease, Department of Internal Medicine, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan;2. Department of Infection Control, E-Da Hospital, Kaohsiung, Taiwan;3. School of Medicine for International Students, I-Shou University, Kaohsiung, Taiwan;4. Faculty of Medicine, School of Medicine, National Yang-Ming University, Taipei, Taiwan;5. Department of Parasitology, Kaohsiung Medical University, Kaohsiung, Taiwan;1. Centre for International Child Health, Department of Paediatrics, Imperial College London, London, UK;2. Vaccines and Immunity Theme MRC Unit The Gambia at London School of Hygiene and Tropical Medicine, Fajara, The Gambia;1. Unité de Pharmacologie Clinique, Institut National de la Santé et de la Recherche Médicale (INSERM) Centre d''Investigation Clinique (CIC) 1406, Centre Hospitalier Universitaire (CHU) Grenoble-Alpes, 38000 Grenoble, France;2. Clinique de Médecine Vasculaire, CHU Grenoble-Alpes, 38000 Grenoble, France;3. Université Grenoble Alpes, Laboratoire Hypoxie PhysioPathologie (HP2), 38000 Grenoble, France;4. INSERM, HP2, 38000 Grenoble, France
Abstract:Leukotrienes (LTs) and prostanoids (Ps) were detected in sputum of patients with chronic bronchitis and/or bronchiectasis (CB/B) using selective superfusion bioassay and radioimmunoassay (RIA) techniques.Analysis of sputum extracts showed a 4-fold increase in the level of LTB4 compared to the cysteinyl-containing LTs (LTC4/LTD4).The measurement of cyclo-oxygenase products (COPs) indicated relatively greater amounts of the vasodilator prostaglandin E2 (PGE2) and prostacyclin (PGI2) compared to the vasoconstrictor prostaglandin F2∝ (PGF2∝) and thromboxane A2 (TxA2) agents (70:30% of total COPs respectively).The presence of eicosanoids (LTs and Ps) in sputum of patients with CB/B suggest that these biologically active substances may act as mediators of bronchoconstriction and inflammation in these diseases.
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