| 定向进化细胞色素P450BM-3的研究 |
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| 引用本文： | 李红梅,梅乐和,Vlada Urlacher,Rolf D.Schmid.定向进化细胞色素P450BM-3的研究[J].生物加工过程,2006,4(1):27-29,34. |
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| 作者姓名： | 李红梅 梅乐和 Vlada Urlacher Rolf D.Schmid |
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| 作者单位： | 1. 浙江大学,化学工程与生物工程系,杭州,310027;浙江大学,宁波理工学院,宁波,315100
2. Institute of Technical Biochemistry University of Stuttgart,Stuttgart,Germany |
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| 基金项目： | 国家自然科学基金(30570411)，中德CSC-DAAD合作科技人员交流PPP项目 |
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| 摘 要： | 以来自于巨大芽孢杆菌的细胞色素P450BM-3为研究对象,采用随机突变和饱和定点突变定向进化技术对P450BM-3进行改造,通过突变体催化靛蓝显色的特性采用活性琼脂平板分析和96微孔板相结合的高通量筛选成功获得了几个具有更高催化性能的突变体。
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| 关 键 词： | 定向进化 细胞色素P450BM-3 靛蓝 |
| 文章编号： | 1672-3678（2006）01-0027-03 |
| 收稿时间： | 2006-01-07 |
| 修稿时间： | 2006-01-07 |
Directed evolution of cytrochrome P450BM-3 |
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| LI Hong-mei,MEI Le-he,Vlada Urlacher,Rolf D.Schmid.Directed evolution of cytrochrome P450BM-3[J].Chinese Journal of Bioprocess Engineering,2006,4(1):27-29,34. |
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| Authors: | LI Hong-mei MEI Le-he Vlada Urlacher Rolf DSchmid |
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| Institution: | 1. Department of Chemical and Biochemical Engineering, Zhengjiang University, Hangzhou 310027, China; 2. Ningbo Institute of Technology, Zhejiang Univerisity, Ningho 315100, China; 3. Institute of Technical Biochemistry University of Stuttgart, Germany, Stuttgart |
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| Abstract: | Directed evolution is a new developed approach to protein modification engineering.Cytochrome P450BM-3 from B.Megaterium was evolved by random mutagenesis and Site-saturation mutagenesis.Some P450BM-3 mutants were obtained by high through-put screening of the combination with the active agar plates with some amount of substrate indole and 96-well microtile plates of catalytic activities according to colourimetric product formation of blue pigment indigo that the P450BM-3 mutants are able to produce. |
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| Keywords: | directed evolution cytochrome P450BM-3 indigo |
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