Characterization of role of the toxR gene in the physiology and pathogenicity of Vibrio alginolyticus |
| |
Authors: | Chen Chang Wang Qing-bai Liu Zhu-Hong Zhao Jing-jing Jiang Xiao Sun Hong-yan Ren Chun-hua Hu Chao-qun |
| |
Affiliation: | (1) The Key Laboratory of Marine Bio-resources Sustainable Utilization, South China Sea Institute of Oceanology, Chinese Academy of Sciences, West Xingang Road 164, Guangzhou, 510301, China; |
| |
Abstract: | toxR, a conserved virulence-associated gene in vibrios, is identified in Vibrio alginolyticus ZJ51-O, a pathogenic strain isolated from diseased fish. To reveal the role of ToxR in the pathogenicity of V. alginolyticus, a deletion mutant was constructed by allelic exchange. The mutant showed the same level of growth in trypticase soy broth (TSB) and iron-limiting condition, as the wild type strain. However, deletion of toxR severely reduced resistance against bile salts and the capability of biofilm formation. Outer-membrane protein (OMP) analysis showed that a 37-kD protein was absent and a 43-kD protein was decreased in the mutant. By MS/MS, the two proteins are identified as the homologues of OmpT and OmpN, respectively. These data suggest that ToxR might have enhanced the bile resistance and biofilm formation through modulating the production of OMP without affecting the ability of iron acquisition and the virulence to the fish via injection. These results indicate that ToxR may assist V. alginolyticus to colonize on the surface of the fish intestine which is crucial for the initiation of the infection, though it may not be involved in the proliferation of the bacteria in the host tissue. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|