Natural killer cell activity in c?liac disease: Effect ofin vitro treatment on effector lymphocytes and/or target lymphoblastoid,myeloid and epithelial cell lines with gliadin |
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Authors: | M A Farré Castany H H Nguyen M Pospí?il P Fri? H Tlaskalová-Hogenová |
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Institution: | (1) Department of Immunology and Gnotobiology, Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20 Prague 4, Czech Republic;(2) Department of Gastroenterology, 1st Faculty of Medicine, Charles University, 121 11 Prague 1, Czech Republic |
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Abstract: | To analyze the possible involvement of natural killer (NK) cell activity in the pathogenetic mechanism of cœliac disease (CD)
we measured the spontaneous cytotoxic cell activity of peripheral blood mononuclear cells (PMNC) from patients with CD and
from healthy donors. No significant differences were found between the NK cell activity of PMNC from healthy donors and from
patients with CD using a standard51Cr release assay. However, a 30-min treatment of PMNC with gliadin inhibited NK cell activity in patients with CD. On the
other hand, a 1-d incubation with gliadin induced cytotoxic cell activity of PMNC against the NK-resistant target cells such
as the epithelial HT-29 and the lymphoblastoid RAJI cell lines, suggesting that activation of PMNC by cultivation with gliadin
can occur. |
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