Electrokinetic study of the reactions of peritoneal macrophages and eosinophils with IgG immune complexes |
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Authors: | Howard R Petty Robert L Folger B R Ware |
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Institution: | (1) Department of Chemistry, Harvard University, 12 Oxford Street, 02138 Cambridge, MA |
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Abstract: | Electrophoretic light scattering (laser Doppler electrophoresis) has been employed to study the effects of guinea pig IgG
immune complexes on the electrophoretic mobility distributions of guinea pig resident peritoneal cells. The resident population
of cells is composed of macrophages (approximately 75%) and eosinophils (approximately 25%). These cells were separated according
to the well-established method of Boyum. Populations of resident macrophages, eosinophils, and the unfractionated samples
were incubated with soluble immune complexes, antigen alone, or antibody alone. The mean mobility of the resident macrophages
decreased approximately 60% when incubated in the presence of immune complexes, although no effect could be discerned in the
presence of antigen or antibody alone. The width of the resulting macrophage mobility distribution was larger than that of
the control distributions, with a broad shoulder on the high-mobility side, indicating a heterogeneous response of the macrophages
to the immune complexes. Eosinophils react in two distinct fashions. One population of eosinophils is present near the control
experiments. The second population reacts in a manner very similar to that of macrophages. This suggest that at least two
populations of eosinophils are present in the unstimulated guinea pig peritoneal cavity. Results that are intermediate between
these two cases are found when unfractionated samples are studied. |
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Keywords: | Electrophoretic light scattering light scattering of cells reacting with IgG macrophage peritoneal eosinophil peritoneal IgG immune complex |
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