Nerve growth factor (NGF) induced differentiation of human neuroblastoma cells |
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| Authors: | K Paveli? S Spaventi |
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| Affiliation: | 1. Department of Neuroregeneration, Netherlands Institute for Neuroscience, Royal Netherlands Academy of Arts and Sciences, Meibergdreef 47, 1105BA Amsterdam, The Netherlands;2. Department of Neurosurgery, Leiden University Medical Center, Albinusdreef 2, 2333ZA Leiden, The Netherlands;3. Department of Clinical and Biological Sciences, Neuroscience Institute of the Cavalieri Ottolenghi Foundation, University of Turin, Turin, Italy;4. Department of Neurology, Leiden University Medical Center, Albinusdreef 2, 2333ZA Leiden, The Netherlands;5. Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam, Vrije Universiteit Amsterdam, De Boelelaan 1105, 1081HV Amsterdam, The Netherlands;1. Janssen Research and Development (RO-R, RL, PL, DH, HM), Titusville, NJ;2. Janssen Research and Development (EW), Beerse, Belgium;3. Janssen Neuroscience Medical Affairs (EJD), Titusville, NJ;4. Janssen Research and Development (YZ, CJ), Fermont, CA;5. Janssen Research and Development (WCD, JBS), San Diego, CA;6. University of Connecticut School of Medicine (DCS), Farmington, CT;7. The Yale Depression Research Program, Yale University (GS), New Haven, CT;1. School of Chemistry and Materials Sciences, Guizhou Integrated Research Center of Polymer Electromagnetic Materials, Key Laboratory of Functional Organic Molecule, Guizhou Education University, Guiyang 550018, China;2. Technology Center for China Tobacco Henan Industrial Limited Company, Zhengzhou, Henan, 450000, China |
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| Abstract: | 1. Nerve growth factor (NGF) induced differentiation of human neuroblastoma cell line. 2. The differentiated cells had a relatively high activity of adenylate cyclase and cyclic AMP phosphodiesterase, and a high intracellular level of cyclic AMP. 3. These cells synthesized a higher amount of met5-o-enkephalin than undifferentiated cells. 4. Undifferentiated cells bound less met5-enkephalin than differentiated cells. The maximum number of [3H]met5-enkephalin receptor sites per mg of membrane protein increased more in differentiated cells. 5. Previous observations taken together with our results suggests that increased intracellular levels of cyclic AMP after treatment with NGF induced differentiation of human neuroblastoma cells. Reversal of undifferentiated tumor cells into the differentiated changes the capacity of synthesis of met5-enkephalin and its interaction with receptors. |
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