Physical wounding-assisted Agrobacterium-mediated transformation of juvenile cotyledons of a biodiesel-producing plant, Jatropha curcas L. |
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Authors: | Naruemon Khemkladngoen Joyce A Cartagena Kiichi Fukui |
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Institution: | (1) Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka 565-0871, Japan;(2) Department of Material and Life Science, Graduate School of Engineering, Osaka University, Osaka 565-0871, Japan; |
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Abstract: | The non-edible plant Jatropha curcas L. is one of the most promising feedstock for sustainable biodiesel production as it is not a source of edible vegetable
oils, produces high amounts of oil (approx. 30–60% in dry seeds) and does not require high-cost maintenance. However, as
with other undomesticated crops, the cultivation of J. curcas presents several drawbacks, such as low productivity and susceptibility to pests. Hence, varietal improvement by genetic
engineering is essential if J. curcas is to become a viable alternative source of biodiesel. There is to date no well-established and efficient transformation system
for J. curcas. In this study, we tested various physical wounding treatments, such as sonication and sand-vortexing, with the aim of developing
an efficient Agrobacterium-mediated transformation for J. curcas. The highest stable transformation rate (53%) was achieved when explants were subjected to 1 min of sonication followed by
9 min of shaking in Agrobacterium suspension. The transformation frequency achieved using this protocol is the highest yet reported for J. curcas. |
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