Cryopreservation of first-stage and infective third-stage larvae of Strongyloides stercoralis |
| |
Authors: | T J Nolan L M Aikens G A Schad |
| |
Affiliation: | University of Pennsylvania School of Veterinary Medicine, Department of Pathobiology, Philadelphia 19104-6050. |
| |
Abstract: | Infective third-stage larvae of Strongyloides stercoralis were frozen over liquid nitrogen and remained infective to dogs when thawed. Successful cryopreservation depended on a 30-60-min incubation in a cryoprotectant (10% DMSO and 10% dextran) before freezing and thawing the frozen larvae into RPMI. First-stage larvae could also be frozen by this method. Thawed first-stage larvae remained viable and continued their development to third-stage larvae, which were shown to be infective to dogs. |
| |
Keywords: | |
|