冬季水淹后洲滩钉螺组织化学与超微结构的变化

实验表明，冬季持续水淹６０天以上，湖沼地区洲滩钉死亡率增高，繁殖力下降。为探索其机制运用组织化学与透射电镜技术观察了受淹钉螺软体组织。结果显示：水淹３０、６０天螺体内糖原含量减少；ＡＬＲ、ｍｇ＾＋＋－ＡＴＰａｓｅ，Ｇ－６Ｐａｓｅ活性下降；ＳＤＨ．ＬＤＨ．ＡＣＰ活性增高；ＣＨＥ活性无明显变化。水淹６０天螺头足部软件 体与肝脏组织细胞肿胀并伴有线体肿胀，嵴断裂或空泡状变，粗面内质网脱颗粒，高尔基体囊状

HISTOCHEMICAL AND ULTRASTRUCTURAL CHANGES OF ONCOMELANIA SNAILS AFTER WINTER FLOODING IN MARSHLAND OF THE YANGTZE RIVER

Our fieed experiments have proven that the oncomelania snails have higher death rates and lower reproductive rates when they were submerged in water for 60-150 days during winter. In order to further evaluate the mechanism, the soft tissuses of the snails submerged for 30-60 days were observed for histochemistry and ultracstructure.The histochemical observations showed that the glycogen content and the activities of ALP,Mg++-ATPase and G-6-Pase were inhibited but those of SDH,LDH,ACP were elevated.These changes of the snails submerged for 60 days were more evidence than those submerged for 30 days. The ultrastructure changes were seen in cuticular cells and liver tissuse of the snail submerged for 60 days.These cells and tissues were swollen with nuclei-enlargement,chromosome agglutination and disorderly arrangement of reticulum of endoplasm etc.