Structural studies on serum albumins under green light irradiation |
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| Authors: | Sorin Comorosan Silviu Polosan Irinel Popescu Elena Ionescu Radu Mitrica Ligia Cristache Alina Elena State |
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| Institution: | (1) National Institute for Advanced Studies in Electrical Engineering, Bucharest, Romania;(2) Department of Optics and Spectroscopy, National Institute of Materials Physics, Bucharest, Romania;(3) Department of Surgery, University of Medicine and Pharmacy, Bucharest, Romania;(4) Department of Biochemistry and Biophysics, University of Medicine and Pharmacy, Bucharest, Romania |
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| Abstract: | This paper presents two new experimental results: the protective effect of green light (GL) on ultraviolet (UV) denaturation
of proteins, and the effect of GL on protein macromolecular structures. The protective effect of GL was revealed on two serum
albumins, bovine (BSA) and human (HSA), and recorded by electrophoresis, absorption, and circular dichroism spectra. The effect
of GL irradiation on protein structure was recorded by using fluorescence spectroscopy and electrophoresis. These new effects
were modeled by quantum-chemistry computation using Gaussian 03W, leading to good fit between theoretical and experimental
absorption and circular dichroism spectra. A mechanism for these phenomena is suggested, based on a double-photon absorption
process. This nonlinear effect may lead to generation of long-lived Rydberg macromolecular systems, capable of long-range
interactions. These newly suggested systems, with macroscopic quantum coherence behaviors, may block the UV denaturation processes. |
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