Comparative analysis of young panicle proteome in thermo-sensitive genic male-sterile rice Zhu-1S under sterile and fertile conditions |
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Authors: | Xiao Xiaojuan Yang Yuanzhu Yang Yuejun Lin Jianzhong Tang Dongying Liu Xuanming |
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Institution: | (1) College of Life Science and Biotechnology, Hunan University, Changsha, China;(2) Academy of Seed Industry of Hunan Yahua, Changsha, China |
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Abstract: | Proteome analysis was carried out to identify the young panicle proteins during different developmental stages under sterile
and fertile conditions. Based on spot quantity and quality, 50 protein spots were analyzed by matrix associated laser desorption/ionization
time of flight mass spectrometry (MALDI-TOF-MS) and 20 spots were identified. Most of these proteins are closely associated
with energy metabolism, protein biosynthesis, cell wall formation and stress responses, which are essential cell activities
to the pollen development. Gene expression analysis of three different proteins by semi-quantitative RT-PCR showed that the
mRNA level was not correlated exactly with the protein level. |
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Keywords: | Proteome Rice semi-quantitative RT-PCR Thermo-sensitive genic male-sterility Young panicle |
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