Establishment of two cell lines from hamster buccal pouch tumors induced by topical 7,12-dimethylbenz(a)anthracene and topical DMBA in conjunction with herpes simplex virus infection |
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| Authors: | N -H Park S D Hudnall D I Paik A D Stock |
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| Institution: | (1) UCLA School of Dentistry, 90024 Los Angeles, California;(2) School of Medicine, 90024 Los Angeles, California;(3) Section of Cytogenetics, City of Hope National Medical Center, 91010 Duarte, California |
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| Abstract: | Summary Two cell lines designated HBPC-1 and HBPC-2 have been established from hamster buccal pouch tumors induced by topical 7,12-dimethylbenz(a)anthracene
(DMBA) and DMBA in conjunction with type 1 herpes simplex virus infection, respectively. The cells are epithelial in morphology,
have a doubling time of approximately 18 h, and require bovine serum for optimal growth. The karyotype is aneuploid, with
several marker chromosomes, and the cells produce squamous cell carcinomas when transplanted into normal hamster pouch tissues.
The study is partly supported by grants from the Smokeless Tobacco Research Council, Inc., #0052 and 0061, and from NIDR #007323. |
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| Keywords: | hamster buccal pouch tumor herpes simplex chemical carcinogen |
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