Extraction of high-molecular-weight DNA from dry root tissue of<Emphasis Type="Italic">Berberis lycium</Emphasis> suitable for RAPD期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Extraction of high-molecular-weight DNA from dry root tissue ofBerberis lycium suitable for RAPD

Authors:	Anil?Kumar author-information" > author-information__contact u-icon-before" > mailto:asaxena@hotmail.com" title=" asaxena@hotmail.com" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author,P.?Pushpangadan,S.?Mehrotra

Affiliation:	(1) Molecular Taxonomy Laboratory, Molecular Biology and Genetic Engineering Division, National Botanical Research Institute, 226001 Lucknow, UP, India;(2) Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, 226001 Lucknow, UP, India

Abstract:	A simple protocol for DNA isolation from dry roots ofBerberis lycium is described. Four-year-old dry roots are used, and the isolated DNA is suitable for analysis by means of restriction enzyme digestion and random amplification of polymorphic DNA (RAPD). The method involves a modified CTAB procedure using 1% PVP to remove polysaccharides and purification using low-melting-temperature agarose. DNA is amplified by means of PCR using 10-mer random primers from Operon Biotechnologies, Inc. (USA), and DNA samples are digested withTaq I,Hind III andEcoR I and examined on agarose gels. The RAPD reaction is performed according to the 1990 protocol by Williams et al.

Keywords:	Berberis DNA isolation dry root
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