Enhanced apoptosis in transformed human lung fibroblasts after exposure to sodium butyrate |
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Authors: | Graham L Thomas Anna Henley Tami C Rowland Animesh Sahai Martin Griffin Paul J Birckbichler |
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Institution: | (1) Oklahoma Medical Research Foundation, Noble Center for Biomedical Research, 825 NE 13th Street, 73104 Oklahoma, Oklahoma City;(2) Department of Life Sciences, The Nottingham Trent University, NG11 8NS Nottingham, England, UK;(3) Department of Urology, University of Oklahoma Health Sciences Centre, 920 Stanton L. Young Blvd. 5SP330, 73190 Oklahoma City, OK |
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Abstract: | Summary Simian virus-transformed human cells, WI-38 VA13A, showed a dose-dependent induction of apoptosis and reduction in cell numbers
after exposure to sodium butyrate. Apoptosis was confirmed by ApopTag staining, isolation of apoptotic envelopes, and immunofluorescent
staining with an antibody specific for apoptotic envelopes. Examination of the cell cultures by phase contrast and fluorescent
microscopy revealed the presence of enlarged cells that displayed a more flattened morphology and morphological changes in
the nucleus of cells exposed to sodium butyrate. Cell proliferation assays showed control and sodium butyrate cultures were
synthesizing DNA and excluded any cytotoxic effects of sodium butyrate. Flow cytometry results indicated an increase in the
number of aneuploid cells following sodium butyrate treatment. There was a decrease in the percentage of cells in G2/M in
the diploid populations, but an increase in the percentage of cells in G2/M in aneuploid populations. This humanin vitro model system suggests a mode of action for the therapeutic effects of sodium butyrate, which have been observed in the topical
treatment of neoplastic cells and reversal of symptoms in ulcerative colitis: namely, the induction of apoptosis. |
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Keywords: | transglutaminase differentiation cell cycle human cells |
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