Measurement of indolebutyric Acid in plant tissues by isotope dilution gas chromatography-mass spectrometry analysis |
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Authors: | Sutter E G Cohen J D |
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Institution: | Department of Pomology, University of California, Davis, California 95616. |
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Abstract: | An internal standard, 13C]indole-2]-indole-3-butyric acid, was synthesized from indole-213C] and was shown to be effective for the quantitative determination of indole-3-butyric acid from plant tissue. When this standard was used along with 13C6]indole-3-acetic acid, both indolic auxins could be quantified from the same tobacco (Nicotiana tabacam) leaf sample by isotope dilution analysis using selected ion monitoring gas chromatography-mass spectrometry for detection. |
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