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Description of bovine major histocompatibility complex class IIa haplotypes using parthenogenetic embryo‐derived cells |
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| Authors: | A. L. A. Sá V. S. G. Leal M. P. C. Schneider O. M. Ohashi E. J. M. Santos P. K. Riggs K. J. Kochan C. A. Gill E. D. Downey J. E. Womack L. C. Skow M. S. Miranda L. Sena |
| Affiliation: | 1. Laboratório de Fecunda??o In Vitro, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil;2. Laboratório de Genética Humana e Médica, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil;3. Laboratório de Polimorfismo de DNA, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil;4. Department of Animal Science, Texas A&M University, College Station, TX, USA;5. Department of Cellular and Molecular Medicine, College of Veterinary Medicine, Texas A&M University, College Station, TX, USA;6. Department of Veterinary Integrative Biosciences, College of Veterinary Medicine, Texas A&M University, College Station, TX, USA |
| Abstract: | In this study, we report an approach to characterize individual BoLA haplotypes using cells from parthenogenetic bovine embryos derived from slaughterhouse ovaries. Eight of the 15 parthenogenetic embryos so obtained had not undergone meiotic recombination on the BoLA region and were suitable to describe BoLA haplotypes. Detailed analysis of the BoLA class IIa region identified seven different class IIa haplotypes, including six not previously described and two new alleles of BoLA‐DQA and one BoLA‐DQB. Our method provided reliable sources of homozygous DNA to describe BoLA haplotypes. |
| Keywords: | BoLA haplotypes DRB3‐DQA‐DQB MHC oocyte activation |