Effect of chitosan elicitation and media components on the production of anthraquinone colorants in madder (Rubia akane Nakai) cell culture |
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Authors: | Je Hyeong Jin Joong Han Shin Ju Hwan Kim In Sik Chung Hyong Joo Lee |
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Institution: | (1) Department of Food Science and Technology and Research Center for New Bio-materials in Agriculture, Seoul National University, Suwon, 441-744, Korea;(2) Department of Genetic Engineering, Kyung Hee University, 449-701 Suwon, Korea |
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Abstract: | To increase the production of anthraquinone colorants in madder (Rubia akane Nakai) cell culture, the effects of elicitation on the colorant production were investigated. Chitosan was the best biotic
elicitor among nine plant derived and microbial derived polysaccharides. When elicited with 25 mg/L chitosan, the total production
was increased approximately two times in a seven-day culture as compared to that in the unelicited cells. Anthraquinone production
was increased in proportion to the contact period up to day 3. Maximum anthraquinone colorants were obtained with 3-day treatment
of chitosan. During chitosan elicitation, the total production was increased 1.3 times in MS medium containing galactose as
compared to that containing sucrose. The degree of deacetylation in chitosan and the use of growth regulator or addition of
precursor did not affect the production of anthraquinone colorants. When madder cells were elicited at optimum condition,
anthraquinone concentration and specific anthraquinone content increased 1.3 times (0.69 g/L) and 2.2 times (0.32 g/g DCW),
respectively. |
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Keywords: | madder (Rubia akane Nakai) anthraquinone colorants elicitation chitosan alizarin |
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