Role of Progesterone Receptor Polymorphisms in the Recurrent Spontaneous Abortions: Indian Case |
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Authors: | Meka Aruna Theeya Nagaraja Sadaranga Andal Surapaneni Tarakeswari Pisapati V S Sirisha Alla G Reddy Kumarasamy Thangaraj Lalji Singh B Mohan Reddy |
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Institution: | 1. Molecular Anthropology Group, Biological Anthropology Unit, Indian Statistical Institute, Hyderabad, India.; 2. Lakshmi Fertility Clinic and Research Centre, Pogathota, Nellore, Andhra Pradesh, India.; 3. Fernandez Hospital, Hyderabad, India.; 4. Centre for Cellular and Molecular Biology, Hyderabad, India.;Health Canada, Canada |
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Abstract: | BackgroundWe attempt to ascertain if the 3 linked single nucleotide polymorphisms (SNPs) of the Progesterone Receptor (PR) gene (exon 1: G 1031 C; S344T, exon 4: G 1978 T; L660V and exon 5: C 2310 T; H770H) and the PROGINS insertion in the intron G, between exons 7 and 8, are associated with Recurrent Spontaneous Abortion (RSA) in the Indian population.Methodology/Principal FindingsA total of 143 women with RSA and 150 controls were sequenced for all the 8 exons looking for the above 3 linked SNPs of the PR gene earlier implicated in the RSA, as well as for any new SNPs that may be possibly found in the Indian population. PROGINS insertion was screened by electrophoresis. We did not find any new mutations, not observed earlier, in our population. Further, we did not find significant role of the *2 allele (representing the mutant allele at the three SNP loci) or the T2 allele (PROGINS insertion) in the manifestation of RSA. We also did not find an LD pattern between each of the 3 SNPs and the PROGINS insertion.Conclusions/SignificanceThe results suggest that the PR gene mutations may not play any exclusive role in the manifestation of RSA, and instead, given significantly higher frequency of the *2 allele among the normal women, we surmise if it does not really confer a protective role among the Indian populations, albeit further studies are required in the heterogeneous populations of this region before making any conclusive statement. |
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