Generation,Purification and Transplantation of Photoreceptors Derived from Human Induced Pluripotent Stem Cells |
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| Authors: | Deepak A. Lamba Andrew McUsic Roli K. Hirata Pei-Rong Wang David Russell Thomas A. Reh |
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| Affiliation: | 1. Department of Biological Structure, University of Washington, Seattle, Washington, United States of America.; 2. Department of Medicine, University of Washington, Seattle, Washington, United States of America.; 3. Department of Biochemistry, University of Washington, Seattle, Washington, United States of America.; 4. Institute for Stem Cells and Regenerative Medicine, University of Washington, Seattle, Washington, United States of America.;Universidade Federal do Rio de Janeiro (UFRJ), Brazil |
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| Abstract: | BackgroundInherited and acquired retinal degenerations are frequent causes of visual impairment and photoreceptor cell replacement therapy may restore visual function to these individuals. To provide a source of new retinal neurons for cell based therapies, we developed methods to derive retinal progenitors from human ES cells.Methodology/Physical FindingsIn this report we have used a similar method to direct induced pluripotent stem cells (iPS) from human fibroblasts to a retinal progenitor fate, competent to generate photoreceptors. We also found we could purify the photoreceptors derived from the iPS cells using fluorescence activated cell sorting (FACS) after labeling photoreceptors with a lentivirus driving GFP from the IRBP cis-regulatory sequences. Moreover, we found that when we transplanted the FACS purified iPSC derived photoreceptors, they were able to integrate into a normal mouse retina and express photoreceptor markers.ConclusionsThis report provides evidence that enriched populations of human photoreceptors can be derived from iPS cells. |
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