The pssB gene product of Rhizobium leguminosarum bv. trifolii is homologous to a family of inositol monophosphatases |
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Authors: | Janczarek M Król J Skorupska A |
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Institution: | Department of General Microbiology, M. Curie-Sklodowska University, Lublin, Poland. |
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Abstract: | The Rhizobium leguminosarum bv. trifolii region encoding pssA and pssB genes was cloned. The pssB gene located upstream of the pssA encoded a 28.36-kDa protein which displayed 97.5% identity with the PssB of R. leguminosarum bv. viciae. Inactivation of the pssB gene by insertion of the lacZ-Gmr cassette resulted in the significant increased production of exopolysaccharide in comparison to the wild-type level. A mutant strain was also defective in nitrogen fixation suggesting a regulatory role of pssB in symbiosis with clover. |
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Keywords: | Candida Neutrophil Respiratory burst reaction Chemiluminescence 2 4-(Hydroxyphenyl)-ethanol Pathogenicity |
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