Cloning and characterization of a highly repetitive fish nucleotide sequence |
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Authors: | U Datta P Dutta R K Mandal |
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Institution: | Department of Biochemistry, Bose Institute, Calcutta, India. |
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Abstract: | We have cloned and sequenced a highly repetitive HindIII fragment of DNA from the common carp Cyprinus carpio. It represents a tandemly repeated sequence with a monomeric unit of 245 bp and comprises 8% of the fish genome. Higher units of this monomer appear as a ladder in Southern blots. The monomeric unit has been sequenced; it is A + T-rich with some direct and some inverse-repeat nucleotide clusters. |
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