Plant regeneration from embryo-derived callus in Phaseolus vulgaris L. (common bean) and P. acutifolius A. Gray (tepary bean) |
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| Authors: | M A Zambre J De Clercq E Vranová M Van Montagu G Angenon W Dillen |
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| Institution: | (1) Laboratorium voor Genetica, Departement Genetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium Fax: +32-9-2645349 E-mail: mamon@gengenp.rug.ac.be, BE |
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| Abstract: | Regeneration-competent callus of Phaseolus vulgaris and P. acutifolius was obtained from mature embryo explants on a medium containing thidiazuron and indole-3-acetic acid. For the P. vulgaris genotype Xan-159, regeneration was achieved from cotyledon explants, but not from embryonic axis explants. Both explants
could be used for the P. acutifolius genotype NI 574 but embryonic axes gave the best results. In-vitro-rooted plantlets of P. acutifolius could readily be established in the greenhouse. For P. vulgaris hardening problems with in-vitro-rooted plantlets could be overcome by means of in vitro grafting. The potential of the described
procedure for obtaining transgenic P. vulgaris plants is discussed.
Received: 31 July 1997 / Revision received: 9 September 1997 / Accepted: 22 December 1997 |
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| Keywords: | Fabaceae In vitro regeneration In vitro grafting Legumes Organogenesis |
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