The effect of phospholipase on the binding of asialoglycorproteins by rat liver plasma membranes

The ability of hepatic plasma membrane to bind desialylated glycoproteins has been shown to be markedly diminished by prior treatment of the membranes with phospholipase A or phospholipase C. In the latter case, the decreased binding capacity was correlated with the loss of membrane-bound phosphate over a wide range of enzyme concentration. However, upon solubilization of the membrane associated binding protein, the sensivity to phospholipase-induced inhibition of binding was eliminated.Additional evidence is presented to support the concept that the observed inhibition is a consequence of non-specific changes in the membrane phospholipids and that phospholipid, per se, does not participate directly in the mechanism of binding.