| 脑源性神经营养因子在大鼠后足切割疼痛中的作用 |
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| 引用本文： | 傅迪,郭曲练,施旭丹,戴茹萍.脑源性神经营养因子在大鼠后足切割疼痛中的作用[J].现代生物医学进展,2010,10(1). |
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| 作者姓名： | 傅迪 郭曲练 施旭丹 戴茹萍 |
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| 作者单位： | 1. 中南大学,湘雅医院麻醉科,湖南,长沙,410011
2. 中南大学,湘雅二医院麻醉科,湖南,长沙,410011 |
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| 基金项目： | 国家自然科学基金(30700790) |
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| 摘 要： | 目的:观察脑源性神经营养因子(BDNF)对大鼠后足切割疼痛的影响。方法:采用纵行切割大鼠后足作为疼痛模型,运用免疫组织化学与免疫荧光双标记方法,观察大鼠后足切割后不同时间点(1-72hr)BDNF在相应节段背根神经节与脊髓内表达的变化。腹腔或鞘内注射BDNF抗体中和内源性BDNF后,以Von Frey尼龙纤维刺激后足行机械痛敏评价。结果:大鼠后足切割后1-24hr内,BDNF在切割侧L42-L5脊髓后角表达明增加,BDNF主要位于后角神经元内与神经末梢,星形胶质细胞与小胶质细胞内未见明显表达;在L42-L5背根神经节,BNDF免疫阳性细胞百分比在切割后1-24hr内也明显增加,增加的主要为大直经神经元;鞘内给予BDNF抗体可明显增加大鼠后足切割后的缩足阈值,而腹腔给予BDNF抗体对大鼠的缩足阈值影响较小。结论:BDNF参与了大鼠后足切割后机械痛敏的过程。
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| 关 键 词： | 脑源性神经营养因子 切割痛 脊髓 背根神经节 大鼠 |
The role of brain derived neurotrohpihc factor (BDNF) in incisional pain in rats |
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| FU Di,GUO Qu-lian,SHI Xu-dan,DAI Ru-ping.The role of brain derived neurotrohpihc factor (BDNF) in incisional pain in rats[J].Progress in Modern Biomedicine,2010,10(1). |
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| Authors: | FU Di GUO Qu-lian SHI Xu-dan DAI Ru-ping |
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| Institution: | FU Di1,GUO Qu-lian1,SHI Xu-dan2,DAI Ru-ping2(1 Anesthesia Department of Xianya Hospital,Central South University,Changsha410011,Hunan,China,2 Anesthsia Department of Xiangya Second Hosiptal,Changsha 410011,Chin) |
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| Abstract: | Objective:To investigate the role of brain derived neutrophic factor in the surgical incision-evoked pain hypersensitivity.Method:Incisional pain rat model was established by a longitudinal incision in right plantar hind paw of isoflurane-anesthetized rats.Dorsal root ganglias(DRG) and spinal cords were removed at various postoperative times(1-72 h).Expression pattern of BDNF was determined by immunohistochemistry and double-labeling immunofluorescence.BDNF monoclonal antibody was administered intrathecally... |
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| Keywords: | brain derived neurotrophic factor(BDNF) spinal cord dorsal root ganglia incisonal pain mechanical allodynia |
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