Cerebral Vulnerability Is Associated with Selective Increase in Extracellular Glutamate Concentration in Experimental Thiamine Deficiency |
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| Authors: | Alan S. Hazell Roger F. Butterworth Antoine M. Hakim |
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| Affiliation: | Brain and Development Research Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, U.S.A.;Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, U.S.A.;Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, U.S.A.;Department of Pathology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, U.S.A. |
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| Abstract: | Abstract: The concentration of apolipoprotein E (apoE), a high-affinity ligand for the low-density lipoprotein receptor, increases dramatically in peripheral nerve following injury. This endoneurial apoE is thought to play an important role in the redistribution of lipids from the degenerating axonal and myelin membranes to the regenerating axons and myelin sheaths. The importance of apoE in nerve repair was examined using mutant mice that lack apoE. We show that at 2 and 4 weeks following sciatic nerve crush, regenerating nerves in apoE-deficient mice were morphologically similar to regenerating nerves in control animals, indicating that apoE is not essential for peripheral nerve repair. Moreover, cholesterol synthesis was reduced in regenerating nerves of apoE-deficient mice as much as in regenerating nerves of control animals. These results suggest that the intraneural conservation and reutilization of cholesterol following nerve injury do not require apoE. |
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| Keywords: | Apolipoprotein E-deficient mice Gene targeting 3-Hydroxy-3-methylglutaryl-CoA reductase Wallerian degeneration Cholesterol |
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