A smooth muscle cell line suitable for the study of voltage sensitive calcium channels |
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| Authors: | U T Rüegg V M Doyle J F Zuber R P Hof |
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| Affiliation: | 1. Department of Physiology, University of Bern, Buehlplatz 5, CH 3012 Bern, Switzerland;2. Department of Emergency Medicine (Notfallzentrum) Inselspital - University of Bern, Freiburgstrasse 10, CH 3010 Bern, Switzerland;1. Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA;2. Department of Medicine, Division of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, CA, USA;3. Boston Medical Center, Boston University Chobanian & Avedisian School of Medicine, Boston University School of Public Health, Boston, MA, USA;4. Department of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, USA;5. Division of Environmental Medicine, Department of Medicine, University of Louisville, Louisville, KY, USA;6. Department of Medicine, Western University, London, ON, Canada |
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| Abstract: | A cell line originating from the fetal rat aorta has been studied with respect to 45Ca2+ uptake. Kinetic experiments showed an initial rapid uptake followed by a slow linear phase; both the initial rate and the maximum uptake were increased in the presence of 55 mM potassium chloride. The calcium channel antagonists, darodipine (PY 108-068) and verapamil, inhibited both the basal and the potassium chloride stimulated uptake. Neither tetrodotoxin nor furosemide affected either basal or depolarisation induced 45Ca2+ uptake. Blockade of the Na+/K+ ATPase by ouabain and of the Ca2+ ATPase by vanadate caused a net increase in cellular 45Ca2+ accumulation. |
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