Use of polyvinyl alcohol as a stabilizer of peroxidase-antibody conjugate for enzyme immunoassay

Summary The activity of a peroxidase-antibody conjugate was greatly stabilized while in solution through the addition of polyvinyl alcohol (PVA). This stabilizing effect was dependent upon the molecular weight and degree of hydrolysis of the PVA used. The concentrations of PVA necessary for maximum stabilization had no adverse effects upon enzyme immunoassay. Thus, the conjugate stabilized by PVA can be used without dilution for enzyme immunoassay, and without need of refrigeration during transport and storage.