Coiled tube membrane bioreactor for cultivation of hybridoma cells producing monoclonal antibodies. |
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| Authors: | J Hagedorn F Kargi |
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| Affiliation: | Department of Chemical Engineering, Washington University, St. Louis, Missouri 63130. |
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| Abstract: | A coiled tube membrane reactor was developed for the cultivation of mouse-mouse hybridoma cells producing monoclonal antibodies. The cell and antibody concentrations obtained in the membrane reactor were higher than that obtained in a batch spinner flask without a membrane. A mathematical model has been developed to describe the membrane transport coupled growth and product formation, and the physical and kinetic constants of the system were determined. |
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